Expression profiling of mouse HPC7 cells

We have expressed mouse Snai1, mutant Snai1 or empty vector in the HPC7 cell line and performed RNA sequencing analysis to identify targets of Snai1 in mouse hematopoietic progenitor cells. Overall design: HPC7 cells were engineered to express either wildtype Snai1, a mutant version of Snai1 that cannot bind Lsd1 or an empty vector control using retroviral transduction. GFP+ cells were sorted after 1 week in culture and RNA was extracted for RNA sequencing.