Two new species of Eleutherodactylus from Western and Central Mexico (Eleutherodactylus jamesdixoni sp. nov., Eleutherodactylus humboldti sp. nov.)

Here, we provide 1) raw and edited recordings of male advertisement calls from two new species of Eleutherodactylus (Syrrhophus) and their sister species, including R scripts used to analyze the data, and 2) photos of preserved specimens from which morphological measurements were collected.

Methods
Raw call recordings were recorded in waveform audio file format (.wav) using a Marantz PMD661 handheld recorder with a Sennheiser shotgun microphone at a sampling rate of 44.1 kHz. Background noise was removed using Audacity version 3.2.1 (Audacity Team, 2022). Filtered recordings were analyzed using Raven Pro 1.6.3 (K. Lisa Yang Center for Conservation Bioacoustics at the Cornell Lab of Ornithology) to create selection tables of call parameters. Morphometric measurements were collected from digital photographs using ImageJ (Rueden et al., 2017).

Audacity Team. 2022. Audacity® software is copyright © 1999-2021 Audacity Team. Website: https://audacityteam.org/.
K. Lisa Yang Center for Conservation Bioacoustics at the Cornell Lab of Ornithology. (2023). Raven Pro: Interactive Sound Analysis Software (Version 1.6.4) [Computer software]. Ithaca, NY: The Cornell Lab of Ornithology. Available from https://ravensoundsoftware.com/.
Rueden CT, Schindelin J, Hiner MC, DeZonia BE, Walter AE, Arena ET, Eliceiri KW. 2017. ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics 18:529. DOI: 10.1186/s12859-017-1934-z.