A Micro-evolutionary Change in Target Binding Sites as a Key Determinant of Ultrabithorax Function in Drosophila [ChIP-seq]

We profiled the genome wide binding of the Ubx protein in Drosophila halteres and identified its direct targets. Analysis of genome wide binding of Ubx helped us identify the role of high affinity motifs in target selection as well as Ubx mediated regulation of target genes in Drosophila halteres. Overall design: We carried out Chromatin immunoprecipitation for the Ubx protein in wing and haltere imaginal discs in Drosophila L3 larvae using a polyclonal antibody raised against the N-terminal region of Drosophila Ubx. Sequencing was carried out on Illumina Hiseq 2500 platform. MACS2 software was used to identify Ubx peaks. All experiments were carried out on three independnt biological replicates.