Data from: Elucidating the functional evolution of heat sensors among Xenopus species adapted to different thermal niches by ancestral sequence reconstruction

Data for Fig 2 & Fig3a-cElectrophysiological data used for Fig 2 and Fig 3a-c. The file include data for current amplitudes and normalized responses obtained by repeated heat stimulation (five times) from Xenopus laevis oocytes expressing TRPV1 from extant and ancestral clawed frog species. A basal current amplitude was obtained in a five-second duration just before each heat stimulation. If the maximum current amplitude was smaller than 0.5 μA, the current data obtained from such oocytes was excluded from analysis since we could not precisely judge the responses of TRPV1 to repeated heat stimulation.Data for Fig2&Fig3a-c.xlsxData for Fig 3eThermal activation thresholds estimated by an Arrhenius plot using data obtained from Xenopus laevis oocytes expressing TRPV1 from extant and ancestral clawed frog species. The data for currents and temperatures were obtained at the first heat stimulation from Xenopus laevis oocytes expressing TRPV1 from extant and ancestral clawed frog species. Then, Arrhenius plots were performed as shown in Fig S2.Data for Fig3e.xlsxData for Fig 5cThermal activation thresholds estimated by an Arrhenius plot using data obtained from Xenopus laevis oocytes expressing TRPA1 from Xenopus species. The data for currents and temperatures were obtained at the first heat stimulation from Xenopus laevis oocytes expressing TRPA1 from Xenopus species. Then, Arrhenius plots were performed as shown in Fig S5.Data for Fig5c.xlsxData for Fig 5d & 5eElectrophysiological data used for Fig 5d & 5e. The file include data for current amplitudes and normalized responses obtained by heat stimulation, then 0.3 mM cinnamaldehyde stimulation from Xenopus laevis oocytes expressing TRPA1 from Xenopus species. A basal current amplitude was obtained in a five-second duration just before each stimulation. The current amplitude elicited by heat stimulation was normalized to that elicited by 0.3 mM cinnamaldehyde stimulation. Dose-dependent responses for Xbo-TRPA1a and Xmu-TRPA1a were obtained by applying different concentrations of cinnamaldehyde (0.01, 0.3, 1, and 2 mM) to Xenopus laevis oocytes expressing TRPA1a from each species. 2 mM cinnamaldehyde was applied to oocytes at the last in all the case to normalize current amplitudes evoked by lower concentrations of cinnamaldehyde.Data for Fig5d&e.xlsxData for Fig 6Electrophysiological data used for Fig 6. The file include data for current amplitudes and normalized responses obtained by heat stimulation, then 0.3 mM cinnamaldehyde stimulation from Xenopus laevis oocytes expressing TRPA1a or TRPA1b from Xenopus laevis. Basal current amplitudes were obtained in a five-second duration just before each stimulation. The current amplitude elicited by heat stimulation was normalized to that elicited by 0.3 mM cinnamaldehyde stimulation. Dose-dependent responses for TRPA1a or TRPA1b from Xenopus laevis were obtained by applying different concentrations of cinnamaldehyde (0.01, 0.3, 1, and 2 mM) to Xenopus laevis oocytes expressing TRPA1a or TRPA1b. 2 mM cinnamaldehyde was applied to oocytes at the last in all the case to normalize current amplitudes evoked by lower concentrations of cinnamaldehyde.Data for Fig6.xlsxNucleotide sequences for Xenopus TRPV1Aligned nucleotide sequences for TRPV1 from extant and ancestral clawed frog species.Nucleotide sequences for Xenopus TRPA1Aligned nucleotide sequences for TRPA1 from Xenopus species.