ExtData_Figure_5_replicates

(A) HEK293T ishXrn1cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with luciferase reporters containing insertions of the indicated lengths from the BCAP31 and SLC7A5 genes, with or without PR8 PA-X. RNA was extracted and used to run 5’ RACE. (B) HEK293T ishXrn1cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with luciferase reporters containing 15 bp insertions from the STOML2 gene with or without mutations to disrupt/restore the hairpin structure. 24 hours post transfection, cells were infected with WT PR8 or PR8-PA(∆X), or mock infected overnight. RNA was then extracted and used to run 5’ RACE. For all gels, the DNA bands were purified and sequenced to confirm their identities. R# --> replicate number; LG### --> experiment number. .sgd files are the original source files acquired with the Syngene Imager.