Sublethal Bioassays: Killifish response to BP related chemicals across a range of salinities simulating the LA coast

Gulf killifish were exposed to sublethal serial dilutions of WAF, CWAF, and Corexit that was previously weathered (1, 4, 16 weeks) in experimental mesocosms at salinities of 4, 12, and 18 ppt. After the 96 h exposure period hepatic tissue and whole body gulf killifsh (0.5g) were collected from treatment dilutions for microsomal and cytosolic preparations. Assays were prepared and completed for whole protein concentrations, Cyp1a (ELISA), NaKATPase (ELISA), Ethoxyresorufin-O-deethylase (EROD), and Glutathione S-transferase (GST) activity. Data from these assays and associated protocols are contained within this dataset, which are referred to as Aim 1B within the original proposal.