Cycloheximide treatment with 15 uM for 2 h

The number and type of synthetic chemicals that are being produced worldwide continues to increase significantly. While these industrial chemicals provide numerous benefits, there is no doubt that some have potential to damage the environment and health. Toxicity must be evaluated and use must be carefully controlled and monitored in order to minimize potential damage. DNA microarray technology has become an important new technique in toxicology. We are using the yeast Saccharomyces cerevisiae as a model organism for toxicological study because it is a simple, fast-growing eukaryote that has been thoroughly characterized. In order to evaluate toxicity by newly synthesized or mixture chemicals, toxicity-induced gene expression alteration profiles by known chemicals should be collected. In our study, cells need to be exposed with same experimental cellular condition, semi lethal (IC50), respectively. In the case of cycloheximide (CAS; 66-81-9), the exposure dose was decided as 15 uM by growth curve with continuously diluted exposure. Cycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms that is recognized as a developmental toxicant and suspected to be a kidney toxicant. Keywords: stress response Series contains triplicate hybridization results from independent biological samples respectively.