Spatial transcriptomics of murine eyelid

Meibomian glands (MGs) are specialized holocrine sebaceous glands embedded in the tarsal plate of the eyelids. The cell heterogeneity of MGs is not well characterized. In this study, we performed a spatial transcriptomic analysis of murine eyelid using the 10X Visium platform. A pair of upper and lower eyelids from an 8-week-old male C57BL/6J mouse was dissected, sectioned at 10 μm, and mounted onto the assay slide containing four capture areas (6.5mm x 6.5mm) with spatially barcoded poly T capture probes. The subsequent procedures were conducted using the 10x Visium Spatial Gene Expression kit, according to the manufacturer’s instructions.