Data from: Comprehensive experimental fitness landscape and evolutionary network for small RNA

Replicate 'm'; FASTQ data for selection rounds 0-4This archive holds FASTQ-formatted sequencing data from replicate 'm' (from May, repl. 1 of 2) of a selection for GTP-binding aptamers; it contains 3 files, each with sequences from 1 or 2 rounds of the selection. See README.txt for more information.may-fastq.tar.bz2Replicate 'j'; FASTQ data for selection rounds 0-4This archive holds FASTQ-formatted sequencing data from replicate 'j' (from July, repl. 2 of 2) of a selection for GTP-binding aptamers; it contains 2 files, each with sequences from 2 or 3 rounds of the selection. See README.txt for more information.july-fastq.tar.bz2Selection Pool StatisticsThis archive includes 6 files which contain statistics regarding the first (May) and second (July) replicate selection pools, including transition probabilities (used to model the selection pools) and the frequency of 5’ and 3’ hexamers (used to correct ligation bias). See README.txt for more information.r0-stats.tarFitness LandscapesThis MS Excel document includes two spreadsheets, each containing the fitness landscape from one of the two replicate selections (May and July). In each landscape, fitness peaks are collections of related sequences and their associated fitness values. Fitness is derived from sequence abundance after correcting for: sequencing errors, bias introduced by sequencing adapter ligation, and non-uniform sequence distribution in the initial pool. Each sequence is listed along with the following information: peak rank, sequence rank (within its peak), label (indicating that peak's rank in both replicates), fitness value (with standard deviation), and edit distance from the peak center. Alignments for each peak are also given. Fitness peaks are presented in descending order of fitness (measured at the peak center), as are sequences within each peak.MayJuly_FitnessLandscapes.xlsx