A cis-regulatory element controls HDAC9 expression to fine-tune inflammasome-dependent chronic inflammation

To understand the underlying molecular mechanism of Hdac9-mediated inflammasome activation and motivated by the molecular scaffolding function of HDAC9, we pursued the hypothesis that HDAC9 may serve as a docking site for cytosolic inflammasome components thereby facilitating its rapid formation upon stimulation. We tested this by performing co-immunoprecipitation experiments coupled with mass spectrometry to assess subunits of the inflammasome that interact with HDAC9.