Replication Data for: Characterization of a droplet microfluidic platform for the enrichment of live and functional clones using a genotype-based read-out

This data depository contains all the data used in the following publication:
Characterization of a droplet microfluidic platform for the enrichment of live and functional clones using a genotype-based read-out

The following data is provided:

- dPCR folder: contains .tiff images of the dPCR experiments to characterize the effect of the copy number on the sensitivity (Table 1 in the paper). The file name corresponds to the sample. Abbreviations: HC = High-copy number, LC = low-copy number, SC = single-copy number, EP = End-point, SP = starting point. The number of positive droplets can be derived from the FAM image, while the total number of droplets can be obtained from the VIC image. For the HC sample, we only have starting and end-point FAM images.

- FACS folder: contains all FACS data. The 20221116_Flowjo_Analyzed_data pdf file contains the analyzed data in FlowJo which was used to determine the fraction of positive droplets. The raw FACS data files (.fsc) have been made available in the FASC_data_files folder. The FACS_sort_parameters folder contains a summary of the sort per sample and per run and was used to obtain how many target droplets were sorted per sample.

- Imagestream folder. Contains the raw Imagestream data of the sample before and after enrichment. (Presort vs Postsort). Imagestream data can be opened and analyzed using FSC Express.

- Data_tables: Excel file containing the calculations and raw data for generating the tables in the paper.

- Data_supplementary_figures: Python file (Jupiter Notebook). Contains the data and code for generating the supplementary figures.