Localization of XAC (Xenopus ADF/cofilin) in Xenopus keratocytes done with immuno-EM. A higher mag view of a localized region of the cell is shown in CIL 24808. Image corresponds to Figure 8g from J C
A single molecule TIRFM assay was employed to measure the kinase activity of purified PI3K molecules on a supported lipid bilayer by counting the number of product PIP3 molecules they produce.