Random genomic sequencing and analysis by the Genolevures Consortium as part of a comparative genomics study of hemiascomycete yeasts. Kluyveromyces marxianus CBS 712

In a large-scale comparative genomics analysis of hemiascomycetes yeasts, the Genolevures Consortium partially sequenced the genomes of 13 yeasts, including that of Kluyveromyces marxianus (also known as Kluyveromyces marxianus var. marxianus) strain CBS 712. For each species, they prepared a random genomic DNA library containing 3- to 5-kb inserts and performed single-pass sequencing (up to 1 kb) of both ends of the inserts, resulting in random sequence tags (RSTs). The Genolevures Consortium generated 2,493 RSTs, totaling 2.3 Mb and representing approximately 0.2X genome coverage, for Kluyveromyces marxianus. They assembled these sequences into contigs and annotated them based on sequential comparisons to DNA and protein sequences from Saccharomyces cerevisiae, protein sequences from other completely sequenced organisms, and protein sequences from all other organisms. The Kluyveromyces marxianus sequences are available in GenBank/EMBL/DDBJ under the accessions AL422385 to AL424880.