Differentially expression profiling in a brain metastasis of a papillary thyroid carcinoma and its technical replicate vs. non-brain metastatic papillary thyroid carcinomas, and primary brain tumors. Homo sapiens

Experiment: Establishment of expression profiles in a brain metastasis from a PTC (RNA processing and hybridization to Affymetrix microarray done twice to yield a technical replicate), in non-brain metastatic, stage III and IV PTCs, and primary brain tumors. Biostatistics analysis identified genes and biofunctions related to the brain metastatic PTC. Overall design: We extracted RNA from tumor specimens and RNA with an RIN > 5 was processed using the recommended processing kits and hybridized to Affymetrix Human Gene 1.0 ST arrays. The CEL files were quantile-normalized and ANOVA was applied to generate differentially expressed probe sets. Probe sets that were significantly associated with the brain metastasis were subjected to biofunctional analysis.