Pleiotropic effects of trisomy and pharmacologic modulation on structural, functional, molecular, and genetic systems in a Down syndrome mouse model

Down syndrome (DS) is characterized by skeletal and brain structural malformations, cognitive impairment, altered hippocampal metabolite concentration, and gene expression imbalance. These alterations were usually investigated separately, and the potential rescuing effects of green tea extracts enriched in epigallocatechin-3-gallate (GTE-EGCG) provided disparate results due to different experimental conditions. Since a holistic evaluation of these systems is missing, we designed a longitudinal experimental setup to follow the simultaneous development of structural, functional, molecular, and genetic alterations in the Ts65Dn mouse model. In this study, we conducted a multi-modal in vivo imaging study using microcomputed tomography (µCT), magnetic resonance imaging (MRI), and magnetic resonance spectroscopy (MRS) to investigate the integrated development of craniofacial shape, BMD, brain volumes, and hippocampal metabolites in wildtype and Ts65Dn mice. Additionally, we evaluated the chan..., , , # Pleiotropic effects of trisomy and pharmacologic modulation on structural, functional, molecular, and genetic systems in a Down syndrome mouse model

[https://doi.org/10.5061/dryad.1rn8pk11r](https://doi.org/10.5061/dryad.1rn8pk11r)

The dataset contains the results of adult cognitive testing, body weight, bone mineral density, concentration of brain hippocampal metabolites, brain volumes, frequency of maternal behavior, neurodevelopmental testing, RNAseq counts, skull shape landmarking files and tibia microarchitecture readouts at different developmental stages for wild-type untreated mice (WT), Ts65Dn untreated mice (TS), wild-type mice treated with GTE-EGCG (WT TREATED) and Ts65Dn mice treated with GTE-EGCG (TS TREATED).

## **Description of the data and file structure**

*Adult cognitive testing:* Results from Open Field (OF), Elevated Plus Maze (EPM), Sociability/Preference for Social Novelty (SPSN), Novel Object Recognition (NOR) and Passive Avoidance (PA) tests. The first sheet...