Moss endogenous peptides, LC-MS/MS

Short (<100 codons) open reading frames (sORFs) are common features of all genomes. Proof of translation of sORFs is an important step towards finding of functional sORF-encoded peptides (SEPs). We verified translation of our predicted sORFs using mass-spectrometry (MS) analysis which is often considered as the gold standard in detection of proteins or peptides in cell. Taking into account the shortage of proteomic methods in identifying small proteins or peptides, in our study we used “peptidomic” dataset - endogenous peptides, extracted from three types of moss cells – gametophores, protonemata and protoplasts