Transcriptome of mature erythrocytes

Purpose: This study identifies the top transcripts expressed in mature erythrocytes and the top transcripts expressed in the polysome fractions of mature erythrocyte lysate Methods: RNA from total erythrocyte lysate and polysome fractions was isolated and purified using GeneJet RNA purification column (Thermo Fisher Scientific). The RNA library was prepared using NEBNext ULTRA II RNA library preparation kit for Illumina. The libraries were sequenced using Illumina Seq. Results: The transcriptome analysis revealed the abundance of mRNAs encoding globins genes, long non-coding RNAs, and micro RNAs. Deciphering the role and functions of these non-coding RNAs in these specialised cells could enable us to understand their biology in greater detail Overall design: RNA transcriptome profiling was done for total mature erythrocyte lysates and polysome fractions using Illumina Seq