Figure 3 Data

3a. High-dimensional analysis of flow cytometry of cells at Day 0 prior to encapsulation and after 3 days in viscous or elastic gels with markers HLA-DR, CD11b, CD141, CD11c, CD1c, CD80, PDL1, and CCR2 gated on live cells, and gated on HLA-DR+CD11b+ live cells. 3b. Relative fluorescence of cell populations in viscous and elastic gels identified by K-means clustering, gated on HLA-DR+CD11b+ live cells. 3c. Flow cytometry of HLA-DR and CD11b with quantification of HLA-DR mean fluorescence intensity (MFI), gated on live cells. 3d. Dendritic cell differentiation markers, CD1c+CD11c+, gated on CD11b+HLA-DR+. 3e. MFI of CCR2+ and fractions of CD80+ and PDL1+ gated on CD11b+HLA-DR+. 3f. Quantification of CD68+ cells, gated on live cells, and CD11c+CD163+ and SLAMF7+ cells, gated on CD11b+HLA-DR+ cells. 3g. Immunofluorescence imaging of pSTAT3 (Tyr705, magenta) with quantification and 3h. Flow cytometry for fraction of CD1c+CD11c+ cells gated on CD11b+HLA-DR+ in viscous and elastic hydrogels with and without STAT3 inhibitor C188-9 (1 uM). 3i. Immunofluorescence imaging of NFKBIZ (IkBz) (magenta) with quantification.