Detection of H2AZ acetylation, total H2AZ, and H4K8 and H4K16 acetylation by CUT&Tag-sequencing

In this study we examined the effects of loss of the MYST histone acetyltransferase TIP60 (KAT5) in mouse embryonic fibroblasts (MEFs), human embryonic kidney cells 293 (HEK293), and human osteosarcoma cells (U2OS) on cell proliferation, BrdU incorporation, cell cycle progression, apoptotic and other forms of cell death, DNA damage, histone acetylation at specific lysine residues and RNA expression levels. This dataset relates to U2OS cells. CUT&Tag was performed on replicate cultures and 100,000 cells per sample, 43,000 human cells each of U2OS with inducible single guide RNA #1 (g1/C9) or inducible single guide RNA #2 (g2/C9) expression and both with constitutive expression of Cas9 enzyme, either treated with doxycycline for 4 days (KO) or untreated (CTL) and 57,000 D. melanogaster cells as a spike-in, in total two replicates of TIP60 deleted cells and two replicates of control cells. Overall design: CUT&Tag-sequencing data were generated on 22 samples of human U2OS cells which are either control (CTL) or TIP60 knock-out (KO) using antibodies to H2AZK4_7_11ac, H2AZK4_7ac, total H2AZ, H4K8ac, H4K16ac, a positive control (H3K27me3) and a negative control (IgG). All samples have Drosophila melanogaser S2 cells spiked in for normalization purposes. Differentially analyses were carried out between the control and KO groups within antibody.