Gene expression of wild-type or GC-A knockout mouse glomeruli treated with high salt diet and aldosterone infusion after uninephrectomy.

Podocytes are integral to maintaining the glomerular filtration barrier and preventing proteinuria. Our previous research underscored the crucial role of podocyte guanylyl cyclase-A (GC-A) in the pathogenesis of severe albuminuria in both systemic and podocyte-specific GC-A knockout mice. In this study, we demonstrate that matrix metalloproteinase-10 (MMP-10) is highly expressed in the glomeruli of GC-A knockout mice and in the kidneys of patients with glomerulonephritis. Systemic GC-A knockout and wild-type mice were performed uninephrectomy before aldosterone infusion and high salt diet for 3 weeks. Total glomerular RNA from these mice was subjected to microarray assay