Specific activity of HpyAV and KpnI.
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aSpecific activity are average values of triplicate experiments for HpyAV and duplicate experiments for KpnI. bComplete cleavage of substrate DNA was not achieved with the highest available concentration of HpyAV. Percentage activity was estimated by comparing the cleavage pattern of the highest concentration of HpyAV with the indicated metal ion to the matching pattern with Ni++ (data not shown). bThe specific activity of KpnI was not determined in Mn++ because star activity appeared before complete cleavage of the substrate DNA was achieved. dSpecific activity of KpnI was not determined in Cu++ because the same incomplete cleavage pattern was observed over a 120-fold difference in enzyme concentration.
创建时间：
2013-02-21
