Genetic engineering of the first functional human cardiomyocyte lines for building clinically relevant atrial fibrillation models

Using conditional immortalization, we generated the first lines of human cardiomyocytes with preserved cardiomyogenic differentiation capacity. These human immortalized Atrial Myocytes (hiAMs) display strict control over proliferation and differentiation, allowing massive (quadrillion-fold) expansion followed by differentiation towards fully functional (i.e. excitable and contractile) human atrial cardiomyocytes. Here, we compared the transcriptome of three hiAM monoclones after differentiation (D12) with hESC-derived AMs (D28) using RNA-sequencing. Total RNA of hiAM monoclones 2.38, 2.52 and 2.90 was sequenced after differentiation (D12). Three hiAM monoclones and four hESC-derived AM replicates were used.