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iTRAQ-Based Proteomics Analysis of Response to Solanum tuberosum Leaves Treated with the Plant Phytotoxin Thaxtomin A

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科学数据银行2022-11-02 更新2026-04-23 收录
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https://www.scidb.cn/detail?dataSetId=aaa56ce5fc2f4ae382a8666c019bbe02
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资源简介:
The S. tuberosum group Phureja DM1–3 were treated with TA and total proteins were extracted from five different plants (pooled together as one sample). The proteomic changes in potato leaves treated with TA were determined using the iTRAQ technique. Protein identification was performed using ProteomeDiscovererTM 2.2 aligned to the NCBInr database (http://www.ncbi.nlm.nih.gov/; accessed on 18 January 2019) and SwissProt/UniProt database (http://www.uniprot.org/; accessed on 18 January 2019) by DIAMOND. Peptide false discovery rate (FDR) analysis ≤0.01 was the filtering parameter. DEPs were identified using a FC (fold change) threshold, and p-values of <0.05 were significant. FC > 1.2 was considered upregulated, and FC < 0.83 was considered downregulated.
提供机构:
College of Life Science, University of Chinese Academy of Sciences; Engineering Laboratory for Advanced Microbial Technology of Agriculture, Chinese Academy of Sciences; Naiqin Zhong; The Enterprise Key Laboratory of Advanced Technology for Potato Fertilizer and Pesticide; State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences
创建时间:
2022-10-19
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