Figure S1 - The Circadian Clock Protein Timeless Regulates Phagocytosis of Bacteria in Drosophila

Tim protein does not regulate AMP gene expression. (A) Basal (uninduced) levels of Diptericin expression were very low and not significantly different at different times of day in wild type and Tim mutants. Every pair-wise combination resulted in p-value greater than 0.05 (not significant) by Mann-Whitney test. (B) Drosomycin expression levels induced by injection of S. pneumoniae or media (BHI) are not circadian-regulated. Wild type and Tim mutants were injected at ZT05 (DAY) or ZT 17 (NIGHT). Pair-wise comparisons of flies injected with S. pneumoniae resulted in p-values greater than 0.05 (not significant) by Mann-Whitney test. Tim mutants injected with media during the day exhibited significantly less Drosomycin expression than wild-type flies injected with media during the day or night or Tim mutants injected at night; all other pair-wise comparisons of flies injected with media did not show significant differences by Mann-Whitney test. S. pneumoniae-infected flies exhibited higher levels of Drosomycin expression than BHI-injected controls; pDiptericin expression were not significantly different after injection of wild type and Tim mutants with E. coli at ZT05 (DAY) or ZT17 (NIGHT). Pair-wise comparisons within the set of flies injected with S. pneumoniae resulted in p-values greater than 0.05 (not significant) by Mann-Whitney test. S. pneumoniae-infected and BHI-injected flies did not exhibit significant differences in Diptericin expression; p>0.05 by Mann-Whitney test. (EPS)