Double phosphorylation of Pto peptide 187–202 and 188–202 upon activation of signalling.
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Prf-3HA, prfK1128A –3HA, prfD1416V –3HA, N-term -3HA (prf1–546 –3HA), Pto-FLAG AvrPto and AvrPtoB were expressed transiently in N. benthamiana under the control of 35S promoter; the total amount of Pto-FLAG was immunoprecipitated using anti-FLAG antibodies. The number of peptides identified with 0, 1, and 2 phosphorylation events is indicated. *ligand-independent HR.
创建时间:
2013-01-31



