Epigenomes of clonal palms with different fruit phenotypes.

Leaf tissue was sampled from normal and mantled clonal palms producing normal and mantled fruits respectively. ChIP-Seq libraries for histone modifications H3K4me3 and H3K27me3 were generated and sequenced on Illumina platform. Genomic regions associated with specific histone marks were identified. Leaf tissue used in this study was sampled from clonal palms derived from a single ortet (mother palm). These 10 clonal palms comprised 5 normal and 5 mantled phenotype palms. Leaf tissues of frond 0 were sampled and used for isolation of chromatin. Chromatin was sheared and a portion of sheared chromatin was set aside for ChIP assay. Sheared chromatin was then immunoprecipitated with H3K4me3- and H3K27me3-mouse monoclonal antibodies (ChIP grade). ChIP-Seq libraries were prepared using DNA from non-immunoprecipitated and ChIP assay. Paired-end sequencing was conducted. Sequencing reads were analysed to identify regions in oil palm genome associated with H3K4me3 and H3K27me3 histone marks.