Thymic tuft cell single-cell mRNA analysis

The goal of the study was to sequence mRNA from tuft cells (identified as CD11c-;CD45-;EpCAM+;IL-25+ using Flare25 reporter mice) in C57BL/6 and Aire-/- mice. The data were used to investigate thymic tuft cell heterogeneity with and without AIRE protein expression. Overall design: Single cells prepared from pooled, digested, thymi were stained for flow cytometry and individual cells were sorted into wells of a 96 well plate containg 5 ul of lysis buffer. As controls, several wells were left blank (0 cells) or 100 cells were sorted.