hyRAD for herbarium genomics in non-model plant species

We combine methods for reduced representation sequencing and hybridization-capture to sequence and compare contemporary and historical plant specimens. We evaluate our strategy with samples of both Arabidopsis thaliana and the non-model plant Cardamine bulbifera. Modern samples were sequenced with ddRAD (A. thaliana: 4 genotypes, 6 biological replicates each; C. bulbifera: 30 samples). Samples obtained from historical herbarium specimens were hyRAD-captured in one or two subsequent rounds (A. thaliana: 10 samples, captured with three bait-types and in two captures, i.e. 10x3x2=60; C. bulbifera: 30 samples, captured twice, i.e. 30x2=60 samples).