Adult-specific trimethylation of histone H3 lysine 4 is prone to dynamic changes with aging in C. elegans somatic cells

In this study, we profiled genome-wide pattern of tri-methylation of lysine 4 on histone H3 (H3K4me3) in the somatic cells of young and old C. elegans to investigate the changes of H3K4me3 during aging. In addition, we profiled H3K4me3 pattern in developmental stage L3. We found that H3K4me3 pattern in L3 is largely maintained in adults and the age-dynamic H3K4me3 are mainly adult stage specific. We profiled gene expression in young and old C. elegans somatic cells with ribo-RNAseq. The changes of H3K4me3 and RNA abundance are positively correlated. Overall design: We performed genome-wide mapping of histone H3 and H3K4me3 in larval stage L3, young adult (D2A) and old adult (D12A) C.elegans somatic cells by ChIP-seq. ribo-RNAseq was performed in young (D2A) and old (D12A) somatic cells to identify RNA expression changes during aging from D2A to D12A.