Development rate of the fresh and cryopreserved 2-cell embryos derived from IVF using superovulated oocytes obtained by IASe treatment.

After IVF between oocytes of C57BL/6 mice and sperm from genetically engineered mice, the 2-cell embryos without (Oocyte donor ID: eCG; 1–3 and IASe; 7, 9, 11) or with cryopreservation (Oocyte donor ID: eCG; 4–6 and IASe; 8, 10, 12) were used for embryo transfer. The results are expressed as mean ± SD. *P < 0.05 compared with eCG in each group of the fresh or cryopreserved embryos. Development rate of the fresh and cryopreserved 2-cell embryos derived from IVF using superovulated oocytes obtained by IASe treatment.