Temporal expression of a) sf32, b) polyhedrin (polh) and c) egt of SfMNPV.

RT-PCR analysis of sf32, polh and egt was performed on total RNA extracted from infected larvae at indicated times post infection (hpi). Transcript amplifications were performed using Sf32.1 and Sf32.2 primers for sf32, Sfpolh.1 and Sfpolh.2 primers for polh and egt.1 and egt.2 primers for egt. RNA was previously treated with DNase and the same amount of RNA was used for sf32, polh and egt amplifications. M indicates RNA from mock-infected larvae (negative control) and C is a positive amplification control of DNA.