Structural and Functional Insights into the Selective Inhibition of Mutant Tau Aggregation by Purpurin and Oleocanthal in Frontotemporal Dementia

Source Data File 1 (Figure 1): Raw thioflavin T (ThT) fluorescence data of tau peptide aggregation is shown in Figure 1b.

Source Data File 2 (Figure 2): Raw sensorgram data for titration curve kinetics of purpurin and oleocanthal binding with wildtype (WT) and mutant tau shown in Figure 2a, b.

Source Data File 3 (Figure 3): Raw thioflavin T (ThT) fluorescence data of tau peptide aggregation in the absence (control) and presence of purpurin or oleocanthal shown in Figure 3a.

Source Data File 4 (Figure 4): Number of nuclei and aggregates used for calculating the normalized aggregate number in HEK293T Tau-RD P301S FRET Biosensor cells shown in Fig. 4b.

Source Data File S1 (Figure S1): Raw fluorescence measurement data of thioflavin T (ThT), purpurin, and oleocanthal shown in Supplementary Figure S1.

Supplementary Dataset S1: MM-GBSA Binding Free Energy Calculations for Purpurin and Oleocanthal with WT and P301L Tau Filaments.

Supplementary Dataset S2: Residue Decomposition Analysis of Purpurin and Oleocanthal Binding to WT and P301L Tau Filaments.