RNA Panel sequencing of isolated microglia of wild-type and TSPO knockout mice treated with diazepam (5mg/kg) or vehicle daily for one week.. RNA Panel sequencing of isolated microglia of wild-type and TSPO knockout mice treated with diazepam (5mg/kg) or vehicle daily for one week.

Diazepam treatment in WT, but not TSPO KO, leads to a loss of dendritic spines in various brain regions. This experiment reveals transcriptional alterations in microglia upon diazepam treatment. Microglia were isolated using a Miltenyi Biotec MACS kit using anti-CD11b beads according to the manufacturer's instructions.Total RNA was isolated using RNeasy Plus Micro Kit and automatically processed on a QIAcube Connect system (QIAGEN, Germany). The library preparation and subsequent sequencing were conducted with TruSeq Targeted RNA Custom Panel Kit, TruSeq Targeted RNA Index Kit A, and MiniSeq Mid Output Kit (Illumina, USA), and sequenced on an Illumina MiniSeq sequencer (Illumina, USA). Reads were aligned to the mouse genome (mm10) using RNA STAR.