Table_1_Integrated Analysis of Metabolome and Transcriptome Data for Uncovering Flavonoid Components of Zanthoxylum bungeanum Maxim. Leaves Under Drought Stress.XLS

Zanthoxylum bungeanum Maxim. leaves (ZBLs) are rich in flavonoids and have become popular in nutrition, foods and medicine. However, the flavonoid components in ZBLs and the mechanism of flavonoid biosynthesis under drought stress have received little attention. Here, we performed an integrative analysis of the metabolome and transcriptome of ZBLs from HJ (Z. bungeanum cv. “Hanjiao”) and FJ (Z. bungeanum cv. “Fengjiao”) at four drought stages. A total of 231 individual flavonoids divided into nine classes were identified and flavones and flavonols were considered the most abundant flavonoid components in ZBLs. The total flavonoid content of ZBLs was higher in FJ; it increased in FJ under drought stress but decreased in HJ. Nine-quadrant analysis identified five and eight differentially abundant flavonoids in FJ and HJ leaves, respectively, under drought stress. Weighted gene correlation network analysis (WGCNA) identified nine structural genes and eight transcription factor genes involved in the regulation of flavonoid biosynthesis. Moreover, qRT-PCR results verified the accuracy of the transcriptome data and the reliability of the candidate genes. Taken together, our results reveal the flavonoid components of ZBLs and document changes in flavonoid metabolism under drought stress, providing valuable information for nutrition value and food utilization of ZBLs.

Zanthoxylum bungeanum Maxim.（香辣木）的叶子富含黄酮类化合物，并在营养、食品及医药领域备受推崇。然而，关于香辣木叶子中黄酮类成分及其在干旱胁迫下黄酮生物合成机制的探讨却鲜有涉及。本研究对HJ（Z. bungeanum cv. “Hanjiao”）和FJ（Z. bungeanum cv. “Fengjiao”）两个品种的香辣木叶在四个干旱阶段下的代谢组和转录组进行了整合分析。共鉴定出231种黄酮类化合物，分为九个类别，其中黄酮类和黄酮醇类被认为是香辣木叶子中最丰富的黄酮成分。FJ中香辣木叶的总黄酮含量高于HJ；在干旱胁迫下，FJ中的黄酮含量增加，而HJ中的黄酮含量则减少。九象限分析识别出FJ和HJ叶片在干旱胁迫下分别有五种和八种差异富集的黄酮类化合物。加权基因相关网络分析（WGCNA）鉴定出九个参与黄酮生物合成调控的结构基因和八个转录因子基因。此外，qRT-PCR结果验证了转录组数据的准确性和候选基因的可靠性。综合而言，本研究揭示了香辣木叶子中的黄酮成分，并记录了干旱胁迫下黄酮代谢的变化，为香辣木的营养价值和食品利用提供了宝贵信息。