Investigating how the SPT2 histone chaperone affects chromatin structure in human osteosarcoma cells.

Histone chaperones play essential roles in the regulation of chromatin structure. Here, we investigated how the SPT2 histone chaperone controls chromatin structure and accessibility in human cells (using U-2 OS osteosarcoma cells as model; these cells also express a H3.3-SNAP transgene). To achieve this, we performed ATAC-seq comparing WT U2OS vs three independent SPT2 KO clones (KO-3, KO-4 and KO-6 Overall design: We performed ATAC-seq on asynchronously growing U-2 OS cells; three independent replicates of the experiment were performed.