Efficiency of cloning of pRNAi-GG at different restriction-ligation times.
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https://figshare.com/articles/dataset/_Efficiency_of_cloning_of_pRNAi_GG_at_different_restriction_ligation_times_/301199
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The number of recombinant colonies for each transformation was counted. Restriction-ligation was performed with continuous incubation at 37°C or for 20–50 cycles (2 min 37°C+5 min16°C). Cloning for pRNAi-GFP and pRNAi-GFP/PDS were performed in duplicate. The negative control was performed without BsaI enzyme. NT, not tested.
For each transformation, 12 clones (when less than 12, pick all) were identified by PCR using vector specific primes P21 and P22, and insert reverse primer P12 (for pRNAi-GFP) or P16 (for pRNAi-GFP/PDS). All the clones showed the expected bands, as part of the results was shown in Fig. 2A.
创建时间：
2012-05-31
