LC-MSMS of DCC and Neuropilin-1 immunoprecipitated samples from Xenopus Laevis brains

During neuronal wiring, extrinsic cues trigger the local translation of specific mRNAs in axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. Here, we analyzed the interactome of endogenous DCC and Neuropilin-1 by analysing immunoprecipitated samples obtained from Xenopus Laevis brains with LC-MSMS. We find that DCC and Neuropilin-1 bind to ribosomal proteins and specific RNA-binding proteins confirming that multiple guidance cue receptors can bind to ribosomes and may use receptor-ribosome coupling to regulate cue-induced local translation in axons.