In vivo experimental evolution of ICC180, a bioluminescent derivative of the mouse enteropathogen Citrobacter rodentium

In these experiments, we set up 10 transmission chains in which <em>C. rodentium</em> transmitted naturally from mouse to mouse. Experiments were performed as described in the collection provided on Protocols.io (1). In addition, nalidixic acid was added as a treatment for five of the transmission chains. To do this, mice were given nalidixic acid in their drinking water at a final concentration of 10 ug/mL. This water was changed every 2-3 days, adding fresh nalidixic acid each time. <br> This dataset contains the weights (g), bacterial viability (as colony forming units [CFU] per gram of stool and as relative light units per gram of stool), and bioluminescence from abdomen and rectum (as photons per second). <br> 1. Read, Patel, &amp; Wiles (2022). Initiating and monitoring natural infection of mice by bioluminescent Citrobacter rodentium. protocols.io. https://www.protocols.io/view/initiating-and-monitoring-natural-infection-of-mic-81wgb6qnnlpk/v1