Supplemental_Figures S1-S4

<b>Figure S1. </b>Representative<b> </b>negative control (secondary antibody only) image for SORL1, showing no non-specific fluorescent binding.<br><b>Figure S2. </b>RNA-sequencing validation of four individual genes, assessed by droplet digital PCR. Boxplots are colored by hibernation season and contain a center line representing the median, boundaries representing the first and third quartiles, and whiskers representing the minimum and maximum values. Boxplots are faceted by sex. The y-axis represents the total copies of the given gene.<br><b>Figure S3. </b>Representative immunohistochemical image of the ARC from male and female AGS at early or late hibernation, using anti-VIM and anti-SORL1 antibodies. Images taken using a 10X objective.<br><b>Figure S4. </b>Estradiol levels for female AGS at early hibernation (EH) and late hibernation (LH). Boxplots are colored by hibernation season and contain a center line representing the median, boundaries representing the first and third quartile, and whiskers representing minimum and maximum values.<br><b>Figure S5.</b> Testosterone levels for male AGS at early hibernation (EH) and late hibernation (LH). Boxplots are colored by hibernation season and contain a center line representing the median, boundaries representing the first and third quartile, and whiskers representing minimum and maximum values.