Potential MDDC reprogramming when exposed to BKPyV
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE154810
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资源简介：
Absence of significant mRNA profile modifications upon BKPyV co-culture in human MDDC suggesting no cell reprogramming. MDDC from four distinct blood donors were analyzed by 3′ digital gene expression (DGE) RNAseq. The libraries were prepared from 10 ng of total RNA. The mRNA poly(A) tails were tagged with universal adapters, specific barcodes and unique molecular identifiers (UMIs) during template switching reverse transcriptase. Barcoded cDNAs from multiple samples were pooled, amplified and tagmented using a transposon-fragmentation. The obtained library was on an Illumina HiSeq 2500. After read counting, DESeq 2 was used to normalize expression.
创建时间：
2021-06-07
