Heterologous expression analysis of wild type Fusarium fujikuroi and Ffvel1deletion strains grown under nitrogen-limiting conditions in liquid media over time.

Investigation of whole genome gene expression level differences of Fusarium fujikuroi between wild-type and a Ffvel1 (velvet) deletion mutant in liquid medium with minimal nitrogen between 24 hr, 72 hr and 120 hr of growth using an array based on a F. verticillioides gene call set. Fusarium fujikuroi produces a number of secondary metabolites including gibberellins, bikaverin, fumonisin and fusarin C that are influenced by nitrogen availability and the velvet global regulatory complex. Overall design: A twelve chip study using total RNA recovered from six cultures of wild-type Fusarium fujikuroi and six cultures of Ffvel1 F. fujikuroi deletion mutant. Each chip measures the expression level of over 13,000 putative genes with twelve 60-mer probes per sequence.

本研究旨在利用基于F. verticillioides基因组的微阵列，探讨野生型稻曲病菌Fusarium fujikuroi与Ffvel1（绒毛）基因敲除突变体在液体培养基中最低氮浓度条件下，在培养24小时、72小时和120小时后全基因组基因表达水平的差异。稻曲病菌产生多种次生代谢产物，包括赤霉素、双脱氧咖啡酸、赭曲霉毒素和fusarin C，这些产物受氮源可用性和绒毛全球调控复合物的影响。总体设计为：一项采用野生型Fusarium fujikuroi和Ffvel1 F. fujikuroi基因敲除突变体各六株培养物中的总RNA，进行的十二芯片研究。每个芯片通过每序列12个60-mer探针，测量超过13,000个候选基因的表达水平。