Proteome analysis of Culter alburnus egg and egg envelope

The changes in the expression of proteins in the eggs of C. alburnus-B and C. alburnus-A at four time points (unfertilized, and 0, 0.5 and 1 h postfertilization, n=3) were quantified by TMT-based proteomics, and the changes in the expression of proteins in the egg envelopes of C. alburnus-B and C. alburnus-A at 1 h after fertilization were quantified by label-free proteomics.