Expression data from leaves of tomato (Solanum lycopersicum L. cv. MicroTom) plants subjected to 1000 Hz sound vibration (at 90 dB for 2 h for 3 consecutive days) and/or inoculated with Pst DC300.

As mechanical stimuli, sound vibrations are known to influence various molecular and physiological processes that can ultimately improve crop performance and yield. Microarray analyses were carried out to investigate the global gene expression changes in MicroTom plants subjected to 1000 Hz sound vibration prior to infection with Pst DC300 to test the effectiveness of sound as a priming agent. Data revealed that 1000 Hz pretreatment is effective in eliciting a transcriptional reprogramming in tomato plants grown under normal conditions, but particularly after Pst DC3000 infection. The protective effect of 1000 Hz pretreatment against Pst DC3000 appeared to be mediated by the upregulation of both unique differentially expressed genes (DEGs) involved in cell wall reinforcement, phenylpropanoid pathway and defensive proteins as well as common DEGs associated with boosting plant basal immunity. Gene expression was analyzed in leaves of Micro-Tom plants subjected to 1000 Hz sound vibration and/or inoculated with Pst DC300 in comparison to untreated controls. Leaves were harvested after 1 day and 3 days of sound treatment and immediately frozen in liquid N2 for subsequent RNA extraction. Three biological replicates (each one including four plants) were considered for each treatment, resulting in a total of 24 samples. Microarrays analysis was performed using the GeneChip® Tomato Gene 1.1 ST Array Strip (Affymetrix).