Differential regulation of flower transpiration during abiotic stress in plants

Soybean plants were subjected to water deficit, heat stress, and combination of water deficit and heat stress along with control condition for 10 days and unopened soybean buds were analysed for differential gene expression compared to control. Soybean plants (16-18 days old) were subjected to water deficit, heat stress, and combination of water deficit and heat stress for 10 days and unopened soybean buds were collected in liquid nitrogen. Total RNA was isolated and mRNA profile were generated by deep sequencing using NovaSeq 6000 PE150.