Composition of developing thrombi obtained from vertical stacks of images collected by multiphoton microscopy of laser induced injuries in mesenteric veins of a mice.

3D image reconstruction of a late stage thrombus is shown in (a) (luminal view) and (b) cross section in a vertical plane (wall is on the left, lumen is on the right). Regions composed mostly of platelets are red, mostly of fibrin - green, composed of platelets and fibrin -yellow; and regions excluding plasma, fibrin and platelets (other material, cells) - black. (c) Shows evolution of the thrombus composition as it stabilizes. Stabilization is associated with decreasing amounts of platelets and increasing amounts of fibrin on the surface.