Heterochronic myeloid cell replacement reveals the local brain environment as key driver of microglia aging

Description

This dataset contains spatial transcriptomics from CosMx platform, for the paper entitled "Heterochronic myeloid cell replacement reveals the local brain environment as key driver of microglia aging." In brief, this paper presents a system that enables in vivo heterochronic myeloid replacement and identified a key signaling pathway involved in aging microglia in the brain. The compressed files contain the raw seurat object output from AtoMx Bioinformatics Platform, provided by Nanostring. Additional raw imaging data or additional metadata for CosMx are available upon request.

Data Manifest

Filename
Contained Objects
Treatment Description
Associated Figures
CosMx Panel

Run1_Young_Old_June_2024.tar.gz

seuratObject_Young_June_2024.RDS

Young HSC into Young animal, replicate #1
Fig. 2; Fig. S4
Mouse 1k Universal

Run1_Young_Old_June_2024.tar.gz

seuratObject_Old_June_2024.RDS

Young HSC into Old animal, replicate #1
Fig. 2; Fig. S4
Mouse 1k Universal

Run2_Young_Old_June_2024.tar.gz

seuratObject_Young_June_2024.RDS

Young HSC into Young animal, replicate #2
Fig. 2; Fig. S4
Mouse 1k Universal

Run2_Young_Old_June_2024.tar.gz

seuratObject_Old_June_2024.RDS

Young HSC into Old animal, replicate #2
Fig. 2; Fig. S4
Mouse 1k Universal

Run3_IFN_Young_Old_Mar_2024.tar.gz

seuratObject_young_12_IFN_Panel.RDS

Young HSC into Young animal, replicate #1
Fig. 3; Fig. S7
Mouse 1k Universal with IFN/Aging gene panel

Run3_IFN_Young_Old_Mar_2024.tar.gz

seuratObject_old_3_IFN_Panel.RDS

Young HSC into Old animal, replicate #1
Fig. 3; Fig. S7
Mouse 1k Universal with IFN/Aging gene panel

Run4_IFN_Young_Old_Mar_2024.tar.gz

seuratObject_young_14_IFN_Panel.RDS

Young HSC into Young animal, replicate #2
Fig. 3; Fig. S7
Mouse 1k Universal with IFN/Aging gene panel

Run4_IFN_Young_Old_Mar_2024.tar.gz

seuratObject_old_5_IFN_Panel.RDS

Young HSC into Old animal, replicate #2
Fig. 3; Fig. S7
Mouse 1k Universal with IFN/Aging gene panel

Run5_Stat1_study_2025.tar.gz

seuratObject_7oc_brain.RDS

Control Young HSC into Old animal, replicate #1
Fig.4; Fig. S11
Mouse 1k Universal with IFN/Aging gene panel

Run5_Stat1_study_2025.tar.gz

seuratObject_4oko_brain.RDS

Stat1 -/- Young HSC into Old animal, replicate #1
Fig.4; Fig. S11
Mouse 1k Universal with IFN/Aging gene panel

Run5_Stat1_study_2025.tar.gz

seuratObject_8oko_brain.RDS

Stat1 -/- Young HSC into Old animal, replicate #2
Fig.4; Fig. S11
Mouse 1k Universal with IFN/Aging gene panel