Transcriptome analysis of Drosophila suzukii reveals molecular mechanisms conferring pyrethroid and spinosad resistance

Drosophila suzukii possess a serrated ovipositor that enables them to lay eggs in soft-skinned, ripening fruits, making this insect a serious threat to berry production. Since its 2008 introduction into North America, growers have used insecticides as the primary approach for D. suzukii management, resulting in detections of insecticide resistance in this pest. This study sought to identify the molecular mechanisms conferring insecticide resistance in these resistant populations. We sequenced the transcriptomes of two pyrethroid- and two spinosad-resistant isofemale lines. In both pyrethroid-resistant lines and one spinosad-resistant line, we identified overexpression of metabolic genes that are implicated in resistance in other insect pests. In the other spinosad-resistant line, we observed an overexpression of cuticular genes that have been linked to resistance. Our findings enabled the development of molecular diagnostics that we used to confirm persistence of insecticide resistance ..., Data generated from Illumina short-read sequencing (PE150)
RNA was extracted from isofemale lines developed from unsprayed Drosophila suzukii collected from populations resistant to either spinosad insecticide or resistant insecticide on either strawberries (S) or caneberries (C).  Female D. suzukii flies were entrained at 25C in 12-hour light:12-hour dark cycles for two full days. On the third day, flies were collected on dry ice sixteen hours after lights-on. This time point was selected because D. suzukii was previously observed to exhibit a low level of cytochrome P450 expression at this time (Hamby et al., 2013). This means any overexpression may be more easily observed. Fly bodies were separated from heads using frozen metal sieves (Newark Wire Cloth Company, Clifton, New Jersey). Eight to ten female bodies were used per sample
Pyrethroid-susceptible lines = S7, S8
Pyrethroid-resistant lines = S3, S4
Spinosad-susceptible lines = C2, C5
Spinosad-resistant lines = C3, C4
Differenti..., , # Transcriptome analysis of Drosophila suzukii reveals molecular mechanisms conferring pyrethroid and spinosad resistance

CA Tabuloc, CR Carlson, F Ganjisaffar, CC Truong, C Chen, KM Lewald, S Hidalgo, N Nicola, C Jones, AA Sial, FG Zalom, JC Chiu

## Data description

Data generated from Illumina short-read sequencing (PE150). RNA was extracted from isofemale lines developed from unsprayed Drosophila suzukii collected from populations resistant to either pyrethroid insecticide (specifically zeta-cypermethrin) or spinosad insecticide on either strawberries (S) or caneberries (C).

Female D. suzukii flies were entrained at 25C in 12-hour light:12-hour dark cycles for two full days. On the third day, flies were collected on dry ice sixteen hours after lights-on (ZT16). This time point was selected because D. suzukii was previously observed to exhibit a low level of cytochrome P450 expression at this time (Hamby et al., 2013). This means any overexpression may be more easily observed. Fly...