Profiling mRNA transcriptomic changes using Affymetrix Clarion D assay in 6 regions of postmortem brains of subjects with alcohol use disorder. Profiling mRNA transcriptomic changes using Affymetrix Clarion D assay in 6 regions of postmortem brains of subjects with alcohol use disorder

Chronic alcohol consumption may alter mRNA transcriptomic changes in reward-related brain regions, leading to neuroadaptations and thus increased risk of AUD. We used the Affymetrix Clariom™ D Assay Array to map mRNA transcriptome profiles in six reward-related regions of postmortem brains of AUD and control subjects and identified differentially expressed mRNAs. Overall design: Total RNAs were extracted from tissue samples dissected from six regions (amygdala, caudate nucleus, cerebellum, hippocampus, prefrontal cortex, and putamen) of postmortem brains of 8 male patients with alcohol use disorder (AUD) and 8 male control subjects. Both AUD patients and control subjects were assessed with DSM-IV. The Affymetrix Clarion D microarray data was processed using the Expression Console (EC) software.