Impact of temperature and hypoxia on the size and survival of aquatic insects

It is crucial to understand impact of the globally rising temperatures on the functional traits of the insects. This is mostly due to the functional traits change, under temperature regime shift, can impact survivability of the individuals and population. Insects´ size is an important functional trait, affected by temperature. It was hypothesized, that many aquatic insects are getting smaller, with the temperature increase, probably due to the hypoxic-induced reduction of metabolic rate.
This dataset contains data on the larvae and pupae size of non-biting midge (Chironomus riparius) and their change in the mesocosm setting, and their change under the impact of different combinations of temperature and hypoxia.
We have compared the body size and survivability of C. riparius in six experimental treatments: three at 20°C, with high, medium and low oxygen concentration and three at 30°C, with the same three levels of oxygen concentrations. To achieve this comparison, we have coll..., Experimental design
Our experimental system utilized laboratory-reared populations of the non-biting midge Chironomus riparius (Meigen, 1804). The mesocosm experiment was conducted from November 10, 2023, to March 23, 2024. Each experimental treatment included four replicate units, with each replicate consisting of a 2.7 L bucket placed inside an insect rearing cage. These cages were housed in a climate-controlled chamber maintained at 20 ± 2 °C, 60 % relative humidity, and an 8:16 hour light:dark cycle. Each bucket was filled with 100 g of clean sand and 2.7 L of dechlorinated water. All replicates for a given treatment were placed within the same cage and climate chamber. Although larvae and pupae developed in isolated water-filled buckets with minimal inter-bucket interaction, replicates within the same cage must be regarded as pseudoreplicates.
We established six treatment conditions: Treatments A, B, and C (\"Lower\" temperature group) were maintained at the chamber's ..., # Impact of temperature and hypoxia on the size and survival of aquatic insects

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We have submitted our raw data (**pupa.csv**,**HC.csv**,**D_treatment_21744379.csv**,**E_treatment_21744382.csv**,**F_tratment_21744381.csv**,**Climate_chamber_control_21744378.csv**, **c_ox.csv**), R project(**hypoxia_temp.Rproj**), R script (**chiro_code1.R**).

## Descriptions

### **pupa.csv**

Raw measurements of the pupal exuviae of *Ch. riparius* derived from the photos at [https://doi.org/10.5281/zenodo.14516968](https://zenodo.org/uploads/14516968) by measuring specimens in Fiji

* *index:* unique numeric index of each observation
* *Treatment*: Experimental treatment group (e.g., temperature and oxygen level combination) assigned to the sample.
* *Folder*: ID of the Folder containing treatment and the date of sampling
* *Area*: The measured area (in pixels or calibrated units) of the specimen from the image.
* *Mean*: The average grayscale pixel intensity within the measured area (used in ima...,