Transcriptome profile of Herbaspirillum seropedicae SmR1 grown in the presence of aromatic compounds. Transcriptome profile of Herbaspirillum seropedicae SmR1 grown in the presence of aromatic compounds
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB9048
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Herbaspirillum seropedicae SmR1 was grown in NFbHPN medium until OD600nm of 0.6, when 5mM phenol or 2,5mM benzoic acid was added to the medium. After 30 minutes of growth, the cells were collected by centrifugation and total RNA was extracted with RiboPureBacteria. Also, a derivative strain from Herbaspirillum seropedicae SmR1 resistant to 1mM phenol (named strain RP) was grown in NFbHPN medium containing 1mM phenol until OD600nm of 0.6, then 5mM phenol was added to the medium. After 30 minutes of growth, the cells were collected by centrifugation and total RNA was extracted with RiboPureBacteria. All samples were depleted for ribossomal RNA e sequenced with Solid plataform.
创建时间：
2016-04-02
